Prevention of Pulmonary Complications of Pneumoperitoneum in Rats
1 Department of Thoracic Surgery, Duzce University School of Medicine, Duzce, Turkey
2 Department of Pathology, Duzce University School of Medicine, Duzce, Turkey
3 Department of Pulmonary Diseases, Duzce University School of Medicine, Duzce, Turkey
4 Department of General Surgery, Duzce University School of Medicine, Duzce, Turkey
5 Department of Anesthesiology and Reanimation, Duzce University School of Medicine, Duzce, Turkey
6 Department of Emergency Medicine, Duzce University School of Medicine, Duzce, Turkey
Journal of Cardiothoracic Surgery 2011, 6:14 doi:10.1186/1749-8090-6-14Published: 8 February 2011
Carbon dioxide (CO2) pneumoperitoneum facilitates the visualization of abdominal organs during laparoscopic surgery. However, the associated increase in intra-abdominal pressure causes oxidative stress, which contributes to tissue injury.
We investigated the ability of the antioxidant and anti-inflammatory drug Erdosteine to prevent CO2 pneumoperitoneum-induced oxidative stress and inflammatory reactions in a rat model.
Fourteen female adult Wistar albino rats were divided into a control group (Group A, n = 7) and an Erdosteine group (Group B, n = 7). Group A received 0.5 cc/day 0.9% NaCl, and Group B received 10 mg/kg/day Erdosteine was administered by gavage, and maintained for 7 days prior to the operation. During the surgical procedure, the rats were exposed to CO2 pneumoperitoneum with an intra-abdominal pressure of 15 mmHg for 30 min. The peritoneal gas was then desufflated. The rats were sacrificed following 3 h of insufflation. Their lungs were removed, histologically evaluated, and scored for intra-alveolar hemorrhage, alveolar edema, congestion, and leukocyte infiltration. The results were statistically analyzed. A value of P < 0.05 was considered statistically significant.
Significant differences were detected in intra-alveolar hemorrhage (P < 0.05), congestion (P < 0.001), and leukocyte infiltration (P < 0.001) in Group A compared with Group B. However, the differences in alveolar edema were not statistically significant (P = 0.698).
CO2 pneumoperitoneum results in oxidative injury to lung tissue, and administration of Erdosteine reduces the severity of pathological changes. Therefore, Erdosteine may be a useful preventive and therapeutic agent for CO2 pneumoperitoneum-induced oxidative stress in laparoscopic surgery.