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This article is part of the supplement: 23rd World Congress of the World Society of Cardio-Thoracic Surgeons: Abstracts

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Volume-activated chloride channel exists in CD133+ lung cancer stem cells

Z Mao1* and X Xu2

Author Affiliations

1 Department of Thoracic Surgery, Renmin Hospital, Wuhan University, Wuhan, Hubei, China

2 Department of Pharmacology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China

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Journal of Cardiothoracic Surgery 2013, 8(Suppl 1):P135  doi:10.1186/1749-8090-8-S1-P135

The electronic version of this article is the complete one and can be found online at:

Published:11 September 2013

© 2013 Mao and Xu; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The study is to research if volume-activated chloride channel (VACC) existed in tumor stem cells.


CD133+ cells were purified by magnetic cell separation (MACS) system from non-small cell lung cancer (NSCLC) cell line A549. The purity was detected by flow cytometry. VACC was recorded with whole-cell patch clamp.


The purity of CD133+ cells isolated from A549 by MACS was 92.14%. And VACC was recorded in the 22/40(55%) CD133+ cells of A549 by whole-cell patch clamp.


VACC exist in CD133+ lung cancer stem cells.