Email updates

Keep up to date with the latest news and content from Journal of Cardiothoracic Surgery and BioMed Central.

This article is part of the supplement: 23rd World Congress of the World Society of Cardio-Thoracic Surgeons: Abstracts

Open Access Poster presentation

Volume-activated chloride channel exists in CD133+ lung cancer stem cells

Z Mao1* and X Xu2

Author Affiliations

1 Department of Thoracic Surgery, Renmin Hospital, Wuhan University, Wuhan, Hubei, China

2 Department of Pharmacology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, China

For all author emails, please log on.

Journal of Cardiothoracic Surgery 2013, 8(Suppl 1):P135  doi:10.1186/1749-8090-8-S1-P135


The electronic version of this article is the complete one and can be found online at: http://www.cardiothoracicsurgery.org/content/8/S1/P135


Published:11 September 2013

© 2013 Mao and Xu; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Background

The study is to research if volume-activated chloride channel (VACC) existed in tumor stem cells.

Methods

CD133+ cells were purified by magnetic cell separation (MACS) system from non-small cell lung cancer (NSCLC) cell line A549. The purity was detected by flow cytometry. VACC was recorded with whole-cell patch clamp.

Results

The purity of CD133+ cells isolated from A549 by MACS was 92.14%. And VACC was recorded in the 22/40(55%) CD133+ cells of A549 by whole-cell patch clamp.

Conclusion

VACC exist in CD133+ lung cancer stem cells.